09.12.2017 // Claude Lachance & Sean Wells, BioRad

Bio-Rad qPCR Seminar- Training: Are you MIQE Compliant?

The MIQE guidelines have helped standardize the way research projects using qPCR are performed. qPCR is the gold standard technique for quantifying levels of RNA and DNA between samples. However, there remains a knowledge gap with respect to the application of rigorous methodology for each step of qPCR to assure reproducible results with accurate conclusions. Proper primer design and validation must be performed and the correct references genes must be selected. The final analysis of the resulting data is not trivial and there are wide variations between labs who typically use EXCEL spreadsheets created by individuals who may or may not understand how to convert the raw data to normalize results to statistical means, standard deviations and their significance with p-values.
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Tuesday Sept. 12 11:30-12:30 BioSci Rm. 3110
Software training- 1:00-3:00 pm BioSci Rm. 3110