02.26// Gordon Chua, Department of Biology, University of Calgary
Transcriptional-regulatory networks establish the gene expression programs responsible for normal growth and disease states. They are composed of interactions between transcription factors and their target genes by direct binding to the promoter. The complete mapping of these networks in model systems by identifying the binding specificities and target genes of each transcription factor remains an important goal to achieve a comprehensive understanding of cellular and organismal systems. The transcriptional-regulatory network of the fission yeast Schizosaccharomyces pombe consists of approximately 100 sequence-specific DNA-binding transcription factors regulating about 5000 genes in the genome. Despite being an extensively-studied model organism, its transcriptional-regulatory network remains substantially incomplete. We have carried out systematic genetics combined with functional genomic approaches such as transcriptomics, genome-wide chromatin immunoprecipitation and synthetic genetic array analysis to functionally characterize S. pombe transcription factors and identify their target genes. These studies have elucidated a novel transcriptional-regulatory network that governs flocculation, implicated new transcription factors and metabolism genes in cell cycle regulation and estimated the functional redundancy of transcription factor gene pairs in S. pombe.
Free pizza lunch BEFORE the talk 11:30-1 pm