Dr. Gian Luca Negri Genome Sciences Centre, BC Cancer Research Centre Unraveling biological complexity through proteomics: applications and challenges
Mass spectrometry has become a commonly used technique to study complex biological processes. Although several strategies now make it possible to measure the proteome with increasing depth and accuracy, several challenges remain. In this talk, I introduce the general workflow of mass spectrometry-based proteomics with a focusing on experimental design, technical and computational considerations. I use examples from flies to humans and highlight the intricacy of integrating mass spectrometry with other high-throughput data.